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nde i restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs nde i restriction enzyme
    Nde I Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nde+i+restriction+enzyme/NdeI/pmc12811641-345-9-13
    Average 99 stars, based on 4828 article reviews
    nde i restriction enzyme - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cloning:

    Article Title: Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion
    Article Snippet: .. Cloning and in vitro cRNA synthesis DNA linearization of Gfp -containing plasmid was carried out using Nde I restriction enzyme (New England BioLabs). .. After DNA purification, in vitro transcription was carried out using an mMessage mMachine T7 kit (Ambion) according to the manufacturer's protocol.

    Article Title: Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons
    Article Snippet: .. Initially, the multiple cloning site from pCR2.1 TOPO was obtained by PCR amplification with primers M13F-NdeI and M13R-NdeI (Table ) and then introduced into pLQ369 by digestion with Nde I restriction enzyme (New England Biolabs) and ligation with T4 DNA ligase (New England Biolabs) to obtain pLQ3poly. .. In a second step, the complete attI1 recombination site was amplified with primers attI1 Avr II F and attI1 ( qacE ) Not I R (Table ) from S. marcescens SCH909 (GCF_015074945.1), cloned into pCR2.1 TOPO using the TOPO TA cloning kit (Invitrogen, CA) to generate pCRattI1, which was then used as substrate for subcloning the attI1 site into the multiple cloning site of pLQ3poly to obtain pMI1-1.

    In Vitro:

    Article Title: Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion
    Article Snippet: .. Cloning and in vitro cRNA synthesis DNA linearization of Gfp -containing plasmid was carried out using Nde I restriction enzyme (New England BioLabs). .. After DNA purification, in vitro transcription was carried out using an mMessage mMachine T7 kit (Ambion) according to the manufacturer's protocol.

    Plasmid Preparation:

    Article Title: Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion
    Article Snippet: .. Cloning and in vitro cRNA synthesis DNA linearization of Gfp -containing plasmid was carried out using Nde I restriction enzyme (New England BioLabs). .. After DNA purification, in vitro transcription was carried out using an mMessage mMachine T7 kit (Ambion) according to the manufacturer's protocol.

    Article Title: Experimental evolution in the cystic fibrosis chemical environment reveals early TCA cycle flux as a central regulator of Mycobacterium abscessus biofilm formation
    Article Snippet: Purification of PCR products was done using the Monarch PCR & DNA Cleanup Kit (New England Biolabs), and the DNA concentration was determined with a NanoDropTM One/OneC Microvolume UV–Vis Spectrophotometer (Thermo ScientificTM) in ng/μL. pSD5hsp60 vectors were extracted from E. coli DH10B cells using the Monarch Plasmid Miniprep Kit (New England Biolabs). .. Restriction enzyme digestion was performed on pSDhsp60 vector using Nde I restriction enzyme (New England Biolabs), which linearized the vector. ..

    Article Title: Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns
    Article Snippet: .. The plasmid was linearized with Nde I restriction enzyme (New England Biolabs) prior to activity studies. ..

    Amplification:

    Article Title: The Predicted Functional Compartmentation of Rice Terpenoid Metabolism by Trans -Prenyltransferase Structural Analysis, Expression and Localization
    Article Snippet: Then, those entry clones were further amplified by KOD FX polymerase (Toyobo, Osaka, Japan) with corresponding primers containing the recognition site of a Nde I restriction enzyme ( ) to allow them to be cloned into pDONR221-Kz- Nde I-sGFP [ ] for the analyses of their subcellular localizations in rice protoplasts. .. The amplified PCR products were digested with Nde I restriction enzyme (NEB, Ipswich, MA, USA) and cloned into pDONR221-Kz- Nde I-sGFP. ..

    Article Title: The role of IL-16 gene polymorphisms in endometriosis
    Article Snippet: .. In addition, the genotyping of the samples for rs11556218 was performed by digesting the 171-bp PCR product with Nde I restriction enzyme (New England BioLabs, Inc.), which digested the DNA that was amplified from the 'G' allele, thus generating two fragments of 147 and 24-bp. ..

    Article Title: Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons
    Article Snippet: .. Initially, the multiple cloning site from pCR2.1 TOPO was obtained by PCR amplification with primers M13F-NdeI and M13R-NdeI (Table ) and then introduced into pLQ369 by digestion with Nde I restriction enzyme (New England Biolabs) and ligation with T4 DNA ligase (New England Biolabs) to obtain pLQ3poly. .. In a second step, the complete attI1 recombination site was amplified with primers attI1 Avr II F and attI1 ( qacE ) Not I R (Table ) from S. marcescens SCH909 (GCF_015074945.1), cloned into pCR2.1 TOPO using the TOPO TA cloning kit (Invitrogen, CA) to generate pCRattI1, which was then used as substrate for subcloning the attI1 site into the multiple cloning site of pLQ3poly to obtain pMI1-1.

    Polymerase Chain Reaction:

    Article Title: The Predicted Functional Compartmentation of Rice Terpenoid Metabolism by Trans -Prenyltransferase Structural Analysis, Expression and Localization
    Article Snippet: Then, those entry clones were further amplified by KOD FX polymerase (Toyobo, Osaka, Japan) with corresponding primers containing the recognition site of a Nde I restriction enzyme ( ) to allow them to be cloned into pDONR221-Kz- Nde I-sGFP [ ] for the analyses of their subcellular localizations in rice protoplasts. .. The amplified PCR products were digested with Nde I restriction enzyme (NEB, Ipswich, MA, USA) and cloned into pDONR221-Kz- Nde I-sGFP. ..

    Article Title: The role of IL-16 gene polymorphisms in endometriosis
    Article Snippet: .. In addition, the genotyping of the samples for rs11556218 was performed by digesting the 171-bp PCR product with Nde I restriction enzyme (New England BioLabs, Inc.), which digested the DNA that was amplified from the 'G' allele, thus generating two fragments of 147 and 24-bp. ..

    Article Title: Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons
    Article Snippet: .. Initially, the multiple cloning site from pCR2.1 TOPO was obtained by PCR amplification with primers M13F-NdeI and M13R-NdeI (Table ) and then introduced into pLQ369 by digestion with Nde I restriction enzyme (New England Biolabs) and ligation with T4 DNA ligase (New England Biolabs) to obtain pLQ3poly. .. In a second step, the complete attI1 recombination site was amplified with primers attI1 Avr II F and attI1 ( qacE ) Not I R (Table ) from S. marcescens SCH909 (GCF_015074945.1), cloned into pCR2.1 TOPO using the TOPO TA cloning kit (Invitrogen, CA) to generate pCRattI1, which was then used as substrate for subcloning the attI1 site into the multiple cloning site of pLQ3poly to obtain pMI1-1.

    Clone Assay:

    Article Title: The Predicted Functional Compartmentation of Rice Terpenoid Metabolism by Trans -Prenyltransferase Structural Analysis, Expression and Localization
    Article Snippet: Then, those entry clones were further amplified by KOD FX polymerase (Toyobo, Osaka, Japan) with corresponding primers containing the recognition site of a Nde I restriction enzyme ( ) to allow them to be cloned into pDONR221-Kz- Nde I-sGFP [ ] for the analyses of their subcellular localizations in rice protoplasts. .. The amplified PCR products were digested with Nde I restriction enzyme (NEB, Ipswich, MA, USA) and cloned into pDONR221-Kz- Nde I-sGFP. ..

    Activity Assay:

    Article Title: Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns
    Article Snippet: .. The plasmid was linearized with Nde I restriction enzyme (New England Biolabs) prior to activity studies. ..

    Ligation:

    Article Title: Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons
    Article Snippet: .. Initially, the multiple cloning site from pCR2.1 TOPO was obtained by PCR amplification with primers M13F-NdeI and M13R-NdeI (Table ) and then introduced into pLQ369 by digestion with Nde I restriction enzyme (New England Biolabs) and ligation with T4 DNA ligase (New England Biolabs) to obtain pLQ3poly. .. In a second step, the complete attI1 recombination site was amplified with primers attI1 Avr II F and attI1 ( qacE ) Not I R (Table ) from S. marcescens SCH909 (GCF_015074945.1), cloned into pCR2.1 TOPO using the TOPO TA cloning kit (Invitrogen, CA) to generate pCRattI1, which was then used as substrate for subcloning the attI1 site into the multiple cloning site of pLQ3poly to obtain pMI1-1.



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